Composite

Part:BBa_K5311012:Design

Designed by: carlos Pérez de Arenaza   Group: iGEM24_UPF-Barcelona   (2024-09-26)


MG_010 + RBS cell free MJ + Cry4Ba + RoxP + His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1686
    Illegal BglII site found at 2916
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 448
    Illegal NgoMIV site found at 3407
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1517
    Illegal BsaI.rc site found at 3620


Design Notes

For this composite part we have codon optimized the CDS for the Cry4Ba toxin to be more compatible with C. acnes, we also introduced the standard prefix and sufixes in our sequence to ensure a proper alignment for the Gibson Assembly


Source

This part was created by using the corresponding sequences on this registry: MG10: Part:BBa_J23119; RBS cell free MJ: Part:BBa_K5311004 ; Cry3Aa: Part:BBa_K5311002; RoxP: Part:BBa_K5311001; and the His-tag corresponds to a sequence of 6 Hystidines


References